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BrainBits LLC hibernate e minus calcium medium
Hibernate E Minus Calcium Medium, supplied by BrainBits LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hibernate+a+medium/hibernate+a+media+with+calcium/bio_rxiv__2025__07__16__665194-288-17-16
Average 90 stars, based on 1 article reviews
hibernate e minus calcium medium - by Bioz Stars, 2026-10
90/100 stars

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Live Cell Imaging:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Transfection:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Virus:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Cell Culture:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Imaging:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Infection:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Recombinant:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Magnetic Cell Separation:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Lysis:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Protease Inhibitor:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Blocking Assay:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

cDNA Synthesis:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Amplification:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

DNA Library Preparation:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Isolation:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).

Red Blood Cell Lysis:

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons
Article Snippet: For live-cell imaging of i 3 Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml −1 BDNF and 10 ng ml −1 NT-3.

Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons.
Article Snippet: For live-cell imaging of i3Neurons, cells were imaged in Hibernate A medium (BrainBits) supplemented with 2% B27, 10 ng ml−1 BDNF and 10 ng ml−1 NT-3.

Article Title: Methods of in vivo evaluation of gene function
Article Snippet: The brains were quickly extracted into ice-cold PBS and cortices were micro-dissected in ice-cold Hibernate A medium (BrainBits, #HA-Lf) with B27 supplement (ThermoFisher, #17504044) under a dissecting microscope.

Article Title: Post-Anesthesia Cognitive Dysfunction in Mice Is Associated with an Age-Related Increase in Neuronal Intracellular [Ca 2+ ]—Neuroprotective Effect of Reducing Intracellular [Ca 2+ ]: In Vivo and In Vitro Studies
Article Snippet: The isolated hippocampus was placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Invitrogen, Waltham, MA, USA).

Article Title: Human brain small extracellular vesicles contain selectively packaged, full-length mRNA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins M-CSF (human recombinant) PeproTech 300–25 HB-EGF (human recombinant) PeproTech 100–47 MACS BSA stock solution Miltenyi Biotec 130-091-376 RNase A Thermo Scientific EN0531 RNaseOUT Invitrogen 10777–019 Cultrex Poly-L-Lysine Fisher Scientific 343820001 Laminin mouse protein, natural Gibco 23017015 RIPA 10X lysis buffer Sigma 20–188 cOmplete, mini, EDTA-free protease inhibitor cocktail Sigma 11836170001 DAKO Universal Antibody Diluent Agilent S302283-2 VECTAShield Vibrance hardset antifade mounting medium with DAPI Vector H-1800 LI-COR intercept (PBS) blocking buffer LI-COR NC1660556 DMEM high glucose Gibco 11965–118 Neurobasal Medium Gibco 21103049 CultureOne supplement 100X Gibco A3320201 GlutaMAX supplement Gibco 35050061 Fetal Bovine Serum VWR 45000–736 Fetal Bovine Serum, exosome-depleted Gibco A2720801 penicillin-streptomycin (10,000U/mL) Gibco 15140122 n-acetyl-l-cysteine bioxtra Sigma A8199-10G Lipopolysaccharides (LPS) from Salmonella enterica serotype Minnesota Sigma L2137-10MG Adenosine 5’-triphosphate disodium salt Sigma A2383-1G B-27 plus supplement (50X) Gibco A3582801 sodium pyruvate Sigma P2256-5G Polyethersulfone 0.2um syringe filter VWR 28145–489 Collagenase type 3 Worthington Biochemical LS004180 Hibernate A Medium BrainBits NC9063747 Critical commercial assays NEBNext single cell/low input cDNA synthesis and amplification module New England Biolabs E6421L Nextera XT DNA library preparation kit Illumina FC-131-1096 IDT for Illumina DNA/RNA UD indexes set A Illumina 20027213 RNeasy micro kit Qiagen 74004 RNeasy mini kit Qiagen 74104 neural tissue dissociation kit - papain Miltenyi Biotec 130-092-628 neural tissue dissociation kit - postnatal neurons Miltenyi Biotec 130-094-802 MACS SmartStrainer, 70 uM Miltenyi Biotec 130-098-462 anti-ACSA-2 MicroBead kit, mouse Miltenyi Biotec 130-097-678 CD11b (microglia) MicroBeads, human and mouse Miltenyi Biotec 130-093-634 neuron isolation kit II, mouse Miltenyi Biotec 130-115-389 red blood cell lysis solution (10x) Miltenyi Biotec 130-094-183 debris removal solution Miltenyi Biotec 130-109-398 LS separation columns Miltenyi Biotec 130-042-401 gentleMACS C tubes Miltenyi Biotec 130-096-334 SMRTbell express template prep kit 2.0 PacBio 100-938-900 (Continued on next page) Cell Reports 43, 114061, April 23, 2024 17

Article Title: Impairment of Glucose Uptake Induced by Elevated Intracellular Ca 2+ in Hippocampal Neurons of Malignant Hyperthermia-Susceptible Mice
Article Snippet: The hippocampus was carefully isolated and placed in ice-cold Hibernate A medium (BrainBits, Springfield, IL, USA) supplemented with B27 and glutamine (Thermo Fisher, Waltham, MA, USA).

Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
Article Snippet: Samples were imaged in a temperature-controlled Tokai Hit microscope stage top incubator (Amuza).

Article Title: Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation.
Article Snippet: Tissue was digested in Hibernate A medium (custom formulation with 50mM glucose and osmolarity adjusted to 280mOsm, BrainBits) supplementedwith 1mM lactic acid (Sigma, L1750), 0.5mMGlutaMAX (ThermoFisher, 35050061) and 2% B27 minus insulin (ThermoFisher, A1895601) containing 20 U/ml papain (Worthington Biochemical Corporation, LS003124) in a shakingwater bath at 34 °C for 30min and dissociated by mechanical trituration through the tips of glass Pasteur pipettes with decreasing diameter (0.9mm,0.7mm, 0.5mm, 0.3mm).



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